Micropropagation of Three Citrus Rootstocks by Tissue Culture

Abstract The experiments were conducted in the Laboratory of Tissue culture/Department Forestry/College Agriculture and Forestry/the University Mosul. To propagate three citrus rootstocks (Citrus aurantium L., Citrumelo swingle , Cariso 35 (C35))In vitro . During Initiation stage, single nodes planted on a solid MS medium supplemented with five concentrations BA (0, 1, 2, 3, 4 mg L -1 ). After four weeks, best significant percentage response for Cariso35 while highest value effect was at 2 (96.67%). No. shoots there are nonsignificant differences, While shoot (1.41) obtained length (2.14cm) compared other rootstocks, so that, 1mg achieved (2.01 cm). At multiplication (2.68shoots), whereas adding gave (3.10). as soon as, (1.03cm) Cariso35, control treatment increase (1.30cm) which differ significantly from treatments. number leaves (11.50 leaves) mgL dominated 11.00 leaves. In rooting stages, 60% cultured MT medium, but non-significant difference between all IBA concentrations. roots 3.28 concentration 1.5 values (2.90 2.24 root) respectively. shortest duration to formed 21.56 18.60 days